CCCCCCCCCCCC CCCCCCCCCCCC CCCC CCCC ooooooo CCCC oo oo nnn CCCC oo oo n n t r i b u t e d Library for the LC ChemStation CCCC oo oo n n CCCC ooooooo CCCC CCCCCCCCCCCC CCCCCCCCCCCC LC ChemStations Revision A.03.xx or higher Date 01/10/94 Macro Contribution #2 Contribution 'Load Maximum Chromatogram from DAD spectral matrix' Revision History ---------------- 1. A.02.xx original version 2. A.03.xx includes installation update. 3. A.06.xx made compatible with the A.05.01 and later revisions data analysis view and signal selection combo box Contribution Purpose -------------------- To extract and display the maximum chromatogram from a set of DAD spectra. Contribution Structure ---------------------- One macro file, 'MaxSign.mac'. Prerequisites ------------- - MS operating system as supported by the revision of the ChemStation in use - The LC ChemStation software, Rev A.03.xx or higher Installation ------------ * Copy the macro to the ChemStation's executable directory, 'C:\CHEM32\CORE' by default. Use --- * Load the macro into the ChemStation using the command ... Macro MaxSign.mac The command self installs into the File menu under the Load sub-menu. After a data file (signal) has been loaded into the ChemStation the menu item can be used to extract the maximum chromatogram and display it. Operation Overview ------------------ The macro checks for data consistency between the loaded chromatogram and a spectral matrix if one exists. If no matrix or the incorrect one is loaded, the DAD spectra are loaded into memory from disc. The macro creates a memory register, that is zero filled, the same size as the chromatographic signal. The matrix rows are scanned for the largest point and these are stored into the appropriate part of the maximum chromatogram memory register. The macro corrects for the differences in data acquisition rates between the chromatographic signal and the spectral matrix that can occur by restricted spectral acquisition modes by extrapolating the last value put into the maximum chromatogram over the missing time interval.