Check your run time under pump parameters flow shouldn't be more than .4ml for ESI .8 ml for APCI Sources.
Save your method to something Unique (LCtest) go to file menu pull down to work list select New
Set up and run worklists 
a Right-click the upper left corner of the worklist.

b Click Add Multiple Samples. The Add Multiple Samples dialog box opens.
c Enter all the information on the Sample Information tab.  
d Click the Sample Position tab to specify the sample vial locations (make sure the specific sample tray type has been configured by right-clicking the autosampler device image).

e Specify the locations, and click OK.
 f To set up the worklist run, right-click the upper left corner, and click Worklist Run Parameters.
 g Click the Page 1 tab.
 h Type the paths for the method and data files.
 i Click the Page 2 tab.
 j Review the information and click OK.
Save the worklist File Pulldown> Worklist> Save as((Test.wkl)
 k To start the run, click the Run Worklist button

Save the worklist File Pulldown> Worklist> Save as((Test.wkl)