<?xml version="1.0" encoding="utf-8"?>
<AcqMethod>
  <Version>1.0</Version>
  <!--Method Properties-->
  <MethodName>PPAS C18 030210.m</MethodName>
  <MethodPath>D:\MassHunter\data\PPA\032210\03221006.d\AcqData\PPAS C18 030210.m</MethodPath>
  <MethodDescription>PPAs using 15 cm X 4.6 mm X 5 um phase</MethodDescription>
  <!--List of Devices-->
  <DeviceList>h-ALS</DeviceList>
  <DeviceList>Quat Pump</DeviceList>
  <DeviceList>Column</DeviceList>
  <DeviceList>MS QQQ</DeviceList>
  <!--Device Specific Setpoints-->
  <WPS>
    <ComponentModelName>h-ALS</ComponentModelName>
    <ComponentModel>G1367B</ComponentModel>
    <ComponentOrdinal>1</ComponentOrdinal>
    <ComponentOptions />
    <Version>1, 0, 0, 1; Base: 0.2.065.0</Version>
    <Stoptime>As Pump</Stoptime>
    <Posttime>Off</Posttime>
    <Delay>0</Delay>
    <CONTACT_1>0</CONTACT_1>
    <CONTACT_2>0</CONTACT_2>
    <CONTACT_3>0</CONTACT_3>
    <CONTACT_4>0</CONTACT_4>
    <ContactsTimetable />
    <Signals>
      <SignalDesc>Temperature</SignalDesc>
      <Store>N/A</Store>
    </Signals>
    <EnableRinse>No</EnableRinse>
    <RinseDrawSpeed>1000</RinseDrawSpeed>
    <RinseEjectSpeed>1000</RinseEjectSpeed>
    <RinseVolume>1.3</RinseVolume>
    <RinseVolume2>2</RinseVolume2>
    <ValveSwitchTime1>0</ValveSwitchTime1>
    <ValveSwitchTime2>0</ValveSwitchTime2>
    <ValveSwitchTime3>0</ValveSwitchTime3>
    <ValveSwitchTime4>0</ValveSwitchTime4>
    <ValveMovements>1</ValveMovements>
    <NRdyTemperatureRange />
    <ThermostatTemperature />
    <DrawPositionDetection>0</DrawPositionDetection>
    <DrawSpeed>200</DrawSpeed>
    <EjectSpeed>200</EjectSpeed>
    <InjVolume>5</InjVolume>
    <DrawPosition>0</DrawPosition>
    <InjectMode>Needle Wash</InjectMode>
    <WashVessel>N/A</WashVessel>
    <OverlapTime>Disable Overlapped Injection</OverlapTime>
    <FlushOutFactor>5</FlushOutFactor>
    <EnableBypass>No</EnableBypass>
    <WaitAfterDraw>0</WaitAfterDraw>
    <WashLocation>FlushPort</WashLocation>
    <WashTime>5</WashTime>
    <WashCycles>N/A</WashCycles>
    <InjProgram />
  </WPS>
  <QuatPump>
    <ComponentModelName>Quat Pump</ComponentModelName>
    <ComponentModel>G1311A</ComponentModel>
    <ComponentOrdinal>1</ComponentOrdinal>
    <ComponentOptions />
    <Version>1.3.15.0; base: 1.3.18.0</Version>
    <Stoptime>15</Stoptime>
    <Posttime>Off</Posttime>
    <Delay>0</Delay>
    <CONTACT_1>0</CONTACT_1>
    <CONTACT_2>0</CONTACT_2>
    <CONTACT_3>0</CONTACT_3>
    <CONTACT_4>0</CONTACT_4>
    <ContactsTimetable />
    <Signals>
      <SignalDesc>Pressure</SignalDesc>
      <Store>0</Store>
    </Signals>
    <Signals>
      <SignalDesc>Flow</SignalDesc>
      <Store>0</Store>
    </Signals>
    <Signals>
      <SignalDesc>Solvent% A</SignalDesc>
      <Store>0</Store>
    </Signals>
    <Signals>
      <SignalDesc>Solvent% B</SignalDesc>
      <Store>0</Store>
    </Signals>
    <Signals>
      <SignalDesc>Solvent% C</SignalDesc>
      <Store>0</Store>
    </Signals>
    <Signals>
      <SignalDesc>Solvent% D</SignalDesc>
      <Store>0</Store>
    </Signals>
    <Signals>
      <SignalDesc>Direction of piston</SignalDesc>
      <Store>0</Store>
    </Signals>
    <StrokeA>Auto</StrokeA>
    <CompressA>50</CompressA>
    <Flow>1</Flow>
    <SolvRatioA>90</SolvRatioA>
    <SolvNameA>0.1% FA in H2O</SolvNameA>
    <PressureMin>0</PressureMin>
    <PressureMax>400</PressureMax>
    <FRamp>100</FRamp>
    <FPTimetable>
      <Time>0</Time>
      <Flow>No Change</Flow>
      <Pressure>No Change</Pressure>
      <SolvRatioB>10</SolvRatioB>
      <SolvRatioC>0</SolvRatioC>
      <SolvRatioD>0</SolvRatioD>
    </FPTimetable>
    <FPTimetable>
      <Time>0.5</Time>
      <Flow>No Change</Flow>
      <Pressure>No Change</Pressure>
      <SolvRatioB>10</SolvRatioB>
      <SolvRatioC>0</SolvRatioC>
      <SolvRatioD>0</SolvRatioD>
    </FPTimetable>
    <FPTimetable>
      <Time>11</Time>
      <Flow>No Change</Flow>
      <Pressure>No Change</Pressure>
      <SolvRatioB>80</SolvRatioB>
      <SolvRatioC>0</SolvRatioC>
      <SolvRatioD>0</SolvRatioD>
    </FPTimetable>
    <FPTimetable>
      <Time>12</Time>
      <Flow>No Change</Flow>
      <Pressure>No Change</Pressure>
      <SolvRatioB>10</SolvRatioB>
      <SolvRatioC>0</SolvRatioC>
      <SolvRatioD>0</SolvRatioD>
    </FPTimetable>
    <FPTimetable>
      <Time>15</Time>
      <Flow>No Change</Flow>
      <Pressure>No Change</Pressure>
      <SolvRatioB>10</SolvRatioB>
      <SolvRatioC>0</SolvRatioC>
      <SolvRatioD>0</SolvRatioD>
    </FPTimetable>
    <PrimChannel>0</PrimChannel>
    <SolvRatioB>10</SolvRatioB>
    <SolvRatioC>Override</SolvRatioC>
    <SolvRatioD>Override</SolvRatioD>
    <SolvNameB>0.1% FA in MeOH</SolvNameB>
    <SolvNameC />
    <SolvNameD />
  </QuatPump>
  <TCC>
    <ComponentModelName>Column</ComponentModelName>
    <ComponentModel>G1316A</ComponentModel>
    <ComponentOrdinal>1</ComponentOrdinal>
    <ComponentOptions>CSV</ComponentOptions>
    <Version>0.2.040.0; Base: 0.2.064.0</Version>
    <Stoptime>As Pump</Stoptime>
    <Posttime>Off</Posttime>
    <Delay>0</Delay>
    <CONTACT_1>0</CONTACT_1>
    <CONTACT_2>0</CONTACT_2>
    <CONTACT_3>0</CONTACT_3>
    <CONTACT_4>0</CONTACT_4>
    <ContactsTimetable />
    <LeftRemoteTemp>0</LeftRemoteTemp>
    <RightRemoteTemp>0</RightRemoteTemp>
    <ColumnPlumbing>0</ColumnPlumbing>
    <LeftTemp>35</LeftTemp>
    <RightTemp>Same as left</RightTemp>
    <RightTempValue>35</RightTempValue>
    <LeftReady>With Any Temp</LeftReady>
    <RightReady>With Any Temp</RightReady>
    <TwoTemperatures>No</TwoTemperatures>
    <ColumnValve>1</ColumnValve>
    <TemperatureTimetable />
  </TCC>
  <MSAcqMethod>
    <version>2007003</version>
    <msInstrument>QQQ</msInstrument>
    <ionSource>ESI</ionSource>
    <tuneFile>atunes.tune.xml</tuneFile>
    <stopMode>ByPumpTime</stopMode>
    <stopTime>1</stopTime>
    <collisionGasOn>true</collisionGasOn>
    <APPILampOn>true</APPILampOn>
    <isTimeFilterEnabled>true</isTimeFilterEnabled>
    <timeFilterPeakWidth>0.07</timeFilterPeakWidth>
    <timeSegments>
      <timeSegment>
        <index>1</index>
        <startTime>0</startTime>
        <diverterValveState>ToMS</diverterValveState>
        <sourceParameters>
          <sourceParameter>
            <id>DGasHeater</id>
            <posPolarityValue>350</posPolarityValue>
            <negPolarityValue>350</negPolarityValue>
          </sourceParameter>
          <sourceParameter>
            <id>DGasFlow</id>
            <posPolarityValue>13</posPolarityValue>
            <negPolarityValue>13</negPolarityValue>
          </sourceParameter>
          <sourceParameter>
            <id>NebulizerPressure</id>
            <posPolarityValue>60</posPolarityValue>
            <negPolarityValue>60</negPolarityValue>
          </sourceParameter>
          <sourceParameter>
            <id>VCap</id>
            <posPolarityValue>4000</posPolarityValue>
            <negPolarityValue>4000</negPolarityValue>
          </sourceParameter>
        </sourceParameters>
        <isDataSaved>true</isDataSaved>
        <scanSegments>
          <scanSegment>
            <index>1</index>
            <ionMode>ESI</ionMode>
            <ionPolarity>Positive</ionPolarity>
            <scanType>MRM</scanType>
            <scanElements>
              <scanElement>
                <index>1</index>
                <compoundName>MPA</compoundName>
                <isISTD>false</isISTD>
                <ms1LowMz>97</ms1LowMz>
                <ms1Res>Unit</ms1Res>
                <ms2LowMz>79</ms2LowMz>
                <ms2Res>Unit</ms2Res>
                <dwell>300</dwell>
                <fragmentor>40</fragmentor>
                <collisionEnergy>15</collisionEnergy>
                <deltaEMV>400</deltaEMV>
              </scanElement>
              <scanElement>
                <index>2</index>
                <compoundName>MPA</compoundName>
                <isISTD>false</isISTD>
                <ms1LowMz>97</ms1LowMz>
                <ms1Res>Unit</ms1Res>
                <ms2LowMz>47</ms2LowMz>
                <ms2Res>Unit</ms2Res>
                <dwell>300</dwell>
                <fragmentor>40</fragmentor>
                <collisionEnergy>25</collisionEnergy>
                <deltaEMV>400</deltaEMV>
              </scanElement>
            </scanElements>
          </scanSegment>
        </scanSegments>
      </timeSegment>
      <timeSegment>
        <index>2</index>
        <startTime>2.5</startTime>
        <diverterValveState>ToMS</diverterValveState>
        <sourceParameters>
          <sourceParameter>
            <id>DGasHeater</id>
            <posPolarityValue>350</posPolarityValue>
            <negPolarityValue>350</negPolarityValue>
          </sourceParameter>
          <sourceParameter>
            <id>DGasFlow</id>
            <posPolarityValue>13</posPolarityValue>
            <negPolarityValue>13</negPolarityValue>
          </sourceParameter>
          <sourceParameter>
            <id>NebulizerPressure</id>
            <posPolarityValue>60</posPolarityValue>
            <negPolarityValue>60</negPolarityValue>
          </sourceParameter>
          <sourceParameter>
            <id>VCap</id>
            <posPolarityValue>4000</posPolarityValue>
            <negPolarityValue>4000</negPolarityValue>
          </sourceParameter>
        </sourceParameters>
        <isDataSaved>true</isDataSaved>
        <scanSegments>
          <scanSegment>
            <index>1</index>
            <ionMode>ESI</ionMode>
            <ionPolarity>Positive</ionPolarity>
            <scanType>MRM</scanType>
            <scanElements>
              <scanElement>
                <index>1</index>
                <compoundName>d7-IMPA</compoundName>
                <isISTD>false</isISTD>
                <ms1LowMz>146</ms1LowMz>
                <ms1Res>Unit</ms1Res>
                <ms2LowMz>98.1</ms2LowMz>
                <ms2Res>Unit</ms2Res>
                <dwell>80</dwell>
                <fragmentor>60</fragmentor>
                <collisionEnergy>5</collisionEnergy>
                <deltaEMV>200</deltaEMV>
              </scanElement>
              <scanElement>
                <index>2</index>
                <compoundName>d7-IMPA</compoundName>
                <isISTD>false</isISTD>
                <ms1LowMz>146</ms1LowMz>
                <ms1Res>Unit</ms1Res>
                <ms2LowMz>80.1</ms2LowMz>
                <ms2Res>Unit</ms2Res>
                <dwell>80</dwell>
                <fragmentor>60</fragmentor>
                <collisionEnergy>30</collisionEnergy>
                <deltaEMV>200</deltaEMV>
              </scanElement>
            </scanElements>
          </scanSegment>
          <scanSegment>
            <index>2</index>
            <ionMode>ESI</ionMode>
            <ionPolarity>Positive</ionPolarity>
            <scanType>MRM</scanType>
            <scanElements>
              <scanElement>
                <index>1</index>
                <compoundName>IMPA</compoundName>
                <isISTD>false</isISTD>
                <ms1LowMz>139</ms1LowMz>
                <ms1Res>Unit</ms1Res>
                <ms2LowMz>97</ms2LowMz>
                <ms2Res>Unit</ms2Res>
                <dwell>80</dwell>
                <fragmentor>60</fragmentor>
                <collisionEnergy>5</collisionEnergy>
                <deltaEMV>200</deltaEMV>
              </scanElement>
              <scanElement>
                <index>2</index>
                <compoundName>IMPA</compoundName>
                <isISTD>false</isISTD>
                <ms1LowMz>139</ms1LowMz>
                <ms1Res>Unit</ms1Res>
                <ms2LowMz>79</ms2LowMz>
                <ms2Res>Unit</ms2Res>
                <dwell>80</dwell>
                <fragmentor>60</fragmentor>
                <collisionEnergy>25</collisionEnergy>
                <deltaEMV>200</deltaEMV>
              </scanElement>
            </scanElements>
          </scanSegment>
          <scanSegment>
            <index>3</index>
            <ionMode>ESI</ionMode>
            <ionPolarity>Positive</ionPolarity>
            <scanType>MRM</scanType>
            <scanElements>
              <scanElement>
                <index>1</index>
                <compoundName>DMMP</compoundName>
                <isISTD>false</isISTD>
                <ms1LowMz>125</ms1LowMz>
                <ms1Res>Unit</ms1Res>
                <ms2LowMz>93</ms2LowMz>
                <ms2Res>Unit</ms2Res>
                <dwell>40</dwell>
                <fragmentor>90</fragmentor>
                <collisionEnergy>15</collisionEnergy>
                <deltaEMV>200</deltaEMV>
              </scanElement>
              <scanElement>
                <index>2</index>
                <compoundName>DMMP</compoundName>
                <isISTD>false</isISTD>
                <ms1LowMz>125</ms1LowMz>
                <ms1Res>Unit</ms1Res>
                <ms2LowMz>63</ms2LowMz>
                <ms2Res>Unit</ms2Res>
                <dwell>40</dwell>
                <fragmentor>90</fragmentor>
                <collisionEnergy>25</collisionEnergy>
                <deltaEMV>200</deltaEMV>
              </scanElement>
            </scanElements>
          </scanSegment>
        </scanSegments>
      </timeSegment>
      <timeSegment>
        <index>3</index>
        <startTime>7.5</startTime>
        <diverterValveState>ToMS</diverterValveState>
        <sourceParameters>
          <sourceParameter>
            <id>DGasHeater</id>
            <posPolarityValue>350</posPolarityValue>
            <negPolarityValue>350</negPolarityValue>
          </sourceParameter>
          <sourceParameter>
            <id>DGasFlow</id>
            <posPolarityValue>13</posPolarityValue>
            <negPolarityValue>13</negPolarityValue>
          </sourceParameter>
          <sourceParameter>
            <id>NebulizerPressure</id>
            <posPolarityValue>60</posPolarityValue>
            <negPolarityValue>60</negPolarityValue>
          </sourceParameter>
          <sourceParameter>
            <id>VCap</id>
            <posPolarityValue>4000</posPolarityValue>
            <negPolarityValue>4000</negPolarityValue>
          </sourceParameter>
        </sourceParameters>
        <isDataSaved>true</isDataSaved>
        <scanSegments>
          <scanSegment>
            <index>1</index>
            <ionMode>ESI</ionMode>
            <ionPolarity>Positive</ionPolarity>
            <scanType>MRM</scanType>
            <scanElements>
              <scanElement>
                <index>1</index>
                <compoundName>PMPA</compoundName>
                <isISTD>false</isISTD>
                <ms1LowMz>361.3</ms1LowMz>
                <ms1Res>Unit</ms1Res>
                <ms2LowMz>97.1</ms2LowMz>
                <ms2Res>Unit</ms2Res>
                <dwell>200</dwell>
                <fragmentor>70</fragmentor>
                <collisionEnergy>15</collisionEnergy>
                <deltaEMV>200</deltaEMV>
              </scanElement>
              <scanElement>
                <index>2</index>
                <compoundName>PMPA</compoundName>
                <isISTD>false</isISTD>
                <ms1LowMz>361.3</ms1LowMz>
                <ms1Res>Unit</ms1Res>
                <ms2LowMz>79.1</ms2LowMz>
                <ms2Res>Unit</ms2Res>
                <dwell>200</dwell>
                <fragmentor>70</fragmentor>
                <collisionEnergy>55</collisionEnergy>
                <deltaEMV>200</deltaEMV>
              </scanElement>
            </scanElements>
          </scanSegment>
          <scanSegment>
            <index>2</index>
            <ionMode>ESI</ionMode>
            <ionPolarity>Positive</ionPolarity>
            <scanType>MRM</scanType>
            <scanElements>
              <scanElement>
                <index>1</index>
                <compoundName>d14-DIMP</compoundName>
                <isISTD>true</isISTD>
                <ms1LowMz>195</ms1LowMz>
                <ms1Res>Unit</ms1Res>
                <ms2LowMz>99</ms2LowMz>
                <ms2Res>Unit</ms2Res>
                <dwell>50</dwell>
                <fragmentor>50</fragmentor>
                <collisionEnergy>5</collisionEnergy>
                <deltaEMV>200</deltaEMV>
              </scanElement>
              <scanElement>
                <index>2</index>
                <compoundName>d14-DIMP</compoundName>
                <isISTD>true</isISTD>
                <ms1LowMz>195</ms1LowMz>
                <ms1Res>Unit</ms1Res>
                <ms2LowMz>80</ms2LowMz>
                <ms2Res>Unit</ms2Res>
                <dwell>50</dwell>
                <fragmentor>50</fragmentor>
                <collisionEnergy>45</collisionEnergy>
                <deltaEMV>200</deltaEMV>
              </scanElement>
            </scanElements>
          </scanSegment>
          <scanSegment>
            <index>3</index>
            <ionMode>ESI</ionMode>
            <ionPolarity>Positive</ionPolarity>
            <scanType>MRM</scanType>
            <scanElements>
              <scanElement>
                <index>1</index>
                <compoundName>DIMP</compoundName>
                <isISTD>false</isISTD>
                <ms1LowMz>181</ms1LowMz>
                <ms1Res>Unit</ms1Res>
                <ms2LowMz>97</ms2LowMz>
                <ms2Res>Unit</ms2Res>
                <dwell>50</dwell>
                <fragmentor>90</fragmentor>
                <collisionEnergy>5</collisionEnergy>
                <deltaEMV>200</deltaEMV>
              </scanElement>
              <scanElement>
                <index>2</index>
                <compoundName>DIMP</compoundName>
                <isISTD>false</isISTD>
                <ms1LowMz>181</ms1LowMz>
                <ms1Res>Unit</ms1Res>
                <ms2LowMz>79</ms2LowMz>
                <ms2Res>Unit</ms2Res>
                <dwell>50</dwell>
                <fragmentor>90</fragmentor>
                <collisionEnergy>45</collisionEnergy>
                <deltaEMV>200</deltaEMV>
              </scanElement>
            </scanElements>
          </scanSegment>
        </scanSegments>
      </timeSegment>
    </timeSegments>
    <instrumentCurves>
      <samplingRate>5</samplingRate>
    </instrumentCurves>
    <chromatograms>
      <chromatogram>
        <index>1</index>
        <chromType>TIC</chromType>
        <tic />
        <label>TIC</label>
        <offset>10</offset>
        <yRange>100000</yRange>
      </chromatogram>
    </chromatograms>
  </MSAcqMethod>
</AcqMethod>