Has anyone ever tried an extraction of bufotenin from anadenanthera peregrina seeds... I was wondering what form of bufotenin is extracted after defatting with water and then mixing that with acetone to isolate the bufotenin?

Has anyone ever tried an extraction of bufotenin from anadenanthera peregrina seeds... I was wondering what form of bufotenin is extracted after defatting with water and then mixing that with acetone to isolate the bufotenin?

I have never done this, but i have snorted these :(...it was long ago tho' and it burns worse then 2c-e! :mad:

Anyways:

http://en.wikipedia.org/wiki/Anadenanthera_peregrina

I do not understand what you mean "what form"? If your speaking of it physically, i presume it is going to be some sticky tar/paste gooey (unless you have some nice equimpent ;) ).

Can you please link me to what extraction your planning to follow?

Thanks

That's my problem the extraction guide I'm following calls for a few things I can't get atm such as calcium hydroxide which alters the bufotenin...Your right that it's physical form is a sticky tar...My question is what the chemical form of bufotenin is that i'm getting from only the defatting and acetone wash... Also how i can get this sticky tar to something easier to handle...my chemistry knowledge is a bit newbish if you couldn't see that already

If you could please post a link/the exact procedure you're planning to follow [as stateofhack asked] it would be really helpful. Anyhow, the bufotenin that you're going to pull after washing the seeds is going to be some sort of organic salt, you really should get some Calcium Hydroxide/Oxide and do it properly, I can't imagine you are totally unable to find the stuff it's not watched or anything. To make the tar easier to handle you could just mix it with something [like powdered seed material] until the consistency improves and what's this you're talking about defating with water?!

That's my problem the extraction guide I'm following calls for a few things I can't get atm such as calcium hydroxide which alters the bufotenin...Your right that it's physical form is a sticky tar...My question is what the chemical form of bufotenin is that i'm getting from only the defatting and acetone wash... Also how i can get this sticky tar to something easier to handle...my chemistry knowledge is a bit newbish if you couldn't see that already

Calcium hydroxide can be bought with no suspicion online or if you look in construction supplies (read the label's) in some of the big "chain" stores. I doubt that the CaOH alters the bufotenin in ANY way! Its just there to basify i belief! But until you don't link me up and i am unable to confirm this point!
As for getting something which is no sticky tar, well chromatography seems the way to go, but i doubt your able to do this (judging by this thread). So i think you will have to "stick" to your sticky resin (hurr hurr u c wut i did thar! ;) ).

what's this you're talking about defating with water?!

lol que?

I'm aware of the simplicity of getting calcium hydroxide i just tend to be impatient nor do i have transportation or any money for that matter to order it or go out and get it.

The extraction guide came from a erowid experience....http://www.erowid.org/experiences/exp.php?ID=64707

Would adding baking soda make it more manageable with only altering the bufotenin to free base bufotenin if its not already pulled in that form....seriously my chemistry knowledge is extremely noobish...thanks for your input though

In the guide it says it alters it to calcium bufotenine which is a more potent and less irritating form of the chemical (by this i mean less nausea and unwelcomed side effects)

In the guide it says it alters it to calcium bufotenine which is a more potent and less irritating form of the chemical (by this i mean less nausea and unwelcomed side effects)

Ahhhh then yes your going to need CaOH, if your aiming for calcium bufotenine. I have never tried the calcium "version" so this would be new to me (tell us how it goes!) and no sodium hydrogencarbonate will not help/replace it.

Can this extraction be done out of order only making it slightly less efficient...meaning would i be able to do the calcium hydroxide step to te acetone pulled bufotenin to still give me calcium bufotenin?

Can this extraction be done out of order only making it slightly less efficient...meaning would i be able to do the calcium hydroxide step to te acetone pulled bufotenin to still give me calcium bufotenin?

Sorry what? I am confused by what your trying to say? Please make yourself a little more clear:)

lol que?

He was talking about defating with water, which doesn't make any sense, how do you defat with a polar solvent? I'm just being nit picky I guess.

Anyhow, there is a lot of debate about the whole calcium bufotenin salt being way stronger and the other forms making you sick etc, interestingly the traditional preparation method involves mixing the stuff with powdered lime so maybe there is some truth there. I've also read some stuff about lipid solubility and ease of passing the BBB - apparently if you don't have a 'freebase' form it's almost useless as a hallucinogen as it can't pass the BBB.

I just had another idea for fixing up your gooey product, why not try washing in a NP solvent [defatting :P]? This should be unnecessary after the water/filter thing to be honest but you've obviously pulled some fats/oils over and the bufotenin shouldn't dissolve in like pet. ether, etc. but the oils will.

He was talking about defating with water, which doesn't make any sense, how do you defat with a polar solvent? I'm just being nit picky I guess.

I know, it was directed at him, not your, sorry was not clear :(

I've already gone through the process of boiling the seed mush taking that extract and washing it with the acetone...do you think i can still make calcium bufotenin by adding the calcium hydroxide to the tarrey resin i got from the acetone wash...This is the reasoning behind my original question what chemical form of bufotenin did i extract with the acetone wash

As far as my statement about defatting with water clearly that shows my knowledge of chemistry...It really goes only as far as reading a few things in a textbook and the informatin you guys provide me here...So would vm p naptha work as the np solvent to defat because ether is unaccessible and i dont have the necessary environment to safely use it as i am aware of it building its own small "atmosphere" on the ground..i'm only using the world for lack of a better one

Yes you can still make the calcium bufotenin with the extract you've prepared, and yes that naphtha will work fine. Have you done any tests on your product yet to make sure it is in fact active? Also let me know what you come up with after the naphtha wash, I'm curious.

P.S. - stateofhack: No worries :)

Is calcium hydroxide my only option to i guess calcify the bufotenin...Or do i have some other option?

Is calcium hydroxide my only option to i guess calcify the bufotenin...Or do i have some other option?

Calcium oxide maybe? You could do a little research and see of other "salts" have been made or tried, it could make it easier for you.

Yea because atm lime/pickling lime are hard to come across it can be done but..I'll see what i can do

Hoping the bufotenin is still active at this point...The process should be done today...I believe i have procurred the sacred lime haha...Will fill in with more info later

Good to hear :)

I'm excited.

Yea because atm lime/pickling lime are hard to come across it can be done but..I'll see what i can do

~sigh~

A majority array of calcium salts are insoluble in water. A large number of other salts are soluble in water...

You know what to do. HARVEST HUMAN BONES NAO!!

Of course....Such a safetly done procedure...Most necessary precautions were taken...of course the only thing that would cause it all to come to a fall would be my mother.... :( I think my point has been made...But don't worry one failure has never made me stop. My theoretical yield and quality comments are still yet to come

my mother.... :(
http://www.totse.com/community/showthread.php?t=2092196&highlight=busted

Of course....Such a safetly done procedure...Most necessary precautions were taken...of course the only thing that would cause it all to come to a fall would be my mother.... :( I

hahha just how old are you please?

Look at my profile...It's not private information but if your truly are concerned then the answer is 15

Look at my profile...It's not private information but if your truly are concerned then the answer is 15

meh, your problem either ways, so how is it proceeding?

New materials have yet to be obtained