View Full Version : DNA Bio help!
lostandconfussed
2008-12-09, 01:58
I need to answer this question for an AP bio class and have no clue what the answer might be.
Human DNA contains introns and exons whereas bacterial DNA does not. Does the human DNA that has been inserted into the bacteria have introns? Explain.
This is relevant to inserting human dna into a bacterial plasmid to produce glucose.
Thanks
Mantikore
2008-12-09, 04:00
this really does belong in Mad Scientists.
or you can try wikipedia.
lol i dunno :confused:
No, it doesn't.
Bacteria don't have the machinery to do RNA-splicing. I believe only eukaryotes and archaea do RNA splicing, though I may be wrong.
I believe the way you would do something like this is to get the gene's mRNA, then treat with reverse transcriptase, *then* use that cDNA to insert into your bacterium.
lostandconfussed
2008-12-10, 01:31
No, it doesn't.
Bacteria don't have the machinery to do RNA-splicing. I believe only eukaryotes and archaea do RNA splicing, though I may be wrong.
I believe the way you would do something like this is to get the gene's mRNA, then treat with reverse transcriptase, *then* use that cDNA to insert into your bacterium.
I thought that codons will only be in the DNA taken from a human, but because the bacteria doe not recognize it, nothing occurs. I guess when I get the essay back we
will know.